The toxicity of endocrine disruptive chemicals is a major concern today (Navaraj and Yasmin, 2012). Understanding the toxic range of the most confirmed endocrine disruptive chemical sources is a must. In line of this objective, a static renewal bioassay test conducted to determine the toxicity of Paper mill effluent, Cypermethrin and Zinc sulphate on the fresh water fish, Oreochromis mossambicus. Fish were exposed to various concentrations of Paper mill effluent, Cypermethrin, Zinc sulphate for 96 hours and the percent mortality was recorded. The 96h LC50 values 6.5%, 0.001ppm, 10.6 ppm of Paper mill effluent, Cypermethrin and Zinc sulphate respectively analysed for Oreochromis mossambicus. The fish is more sensitive to pesticide, Cypermethrin than effluent or zinc metal.